Analysis of Samples of Clinical and Alimentary Interest with by Emilia Witkowska Nery PDF

By Emilia Witkowska Nery

ISBN-10: 3319286714

ISBN-13: 9783319286716

ISBN-10: 3319286722

ISBN-13: 9783319286723

This booklet offers major units of paper-based analytical platforms. the 1st set is a platform for the research of glucose, ldl cholesterol and uric acid in organic samples, and the second one set is a state-of-the-art digital tongue procedure for the research of drinks (mineral water, beer, wine). This thesis additionally presents an intensive evaluate of 33 equipment of enzyme immobilization on paper which were evaluated to augment the garage balance of the proposed approach for biomarker detection. From a pragmatic standpoint, this thesis covers a various set of subject matters concerning paper-based sensing, together with colorimetric and electrochemical detection tools, varied units of structure (spot-tests, lateral and tangential circulate assays), equipment of fabrication (wax printing, slicing, impregnation with polymers), measurements in desk bound and circulation stipulations in addition computing device modeling of proposed structures and complex facts research utilizing chemometric strategies. This publication turns out to be useful for PhD scholars operating during this or a comparable box who require certain information regarding technique and history to this learn.

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1007/s10404-014-1340-z 104. Shin JH, Park J, Kim SH, Park J-K (2014) Programmed sample delivery on a pressurized paper. Biomicrofluidics 8:054121. 4899773 105. Giokas DL, Tsogas GZ, Vlessidis AG (2014) Programming fluid transport in paper-based micro fluidic devices using razor-crafted open channels 106. Noh H, Phillips ST (2010) Fluidic timers for time-dependent, point-of-care assays on paper. Anal Chem 82:8071–8078. 1021/ac1005537 107. Barry L, Tinny L, Elain F et al (2013) Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics.

Diabet Med 26:197–210. 2008. x References 21 93. Allen MP, DeLizza A, Ramel U et al (1990) A noninstrumented quantitative test system and its application for determining cholesterol concentration in whole blood. Clin Chem 36:1591–1597 94. Allen MP (1995) Laminated assay device. 15:1–15 95. Martinez AW, Phillips ST, Butte MJ, Whitesides GM (2007) Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew Chemie - Int Ed 46:1318–1320. 200603817 96. Dungchai W, Chailapakul O, Henry CS (2010) Use of multiple colorimetric indicators for paper-based microfluidic devices.

2), however the buffer capacity and the initial pH of the sample have to be taken into account. Another possibility is the quantification of hydrogen peroxide formed during the enzymatic reaction [11]. g. from horseradish), is used to drive the oxidation of a chromophore. Commercial coupled systems include chromophores such as: 28 2 Analysis of Glucose, Cholesterol and Uric Acid phenol/4-aminoantipyrine, amplex red (10-acetyl-3,7-dihydroxyphenoxazine) and o-dianisidine/sulfuric acid [1]. In the case of electrochemical enzymatic sensing both enzyme families, oxidases and dehydrogenases are widely used for electrooxidation of glucose.

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