By Martin C. Windrow
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Endocr. 33_, 358, (1971); B . E . P . Murphy, Acta Endocr. Suppl. 147, 37, (1970); S. Furuyama, D . M . A. Nugent, Steroids 16, 416, (1970). Table 1 Competitive protein-binding analysis for androgens Using plasma proteins Using antibodies Testosterone Dihydr ote st o s te rone Androstenedione ( —>» testosterone) Dehydroepiandrosterone (—>> 5-androstenediol) Testosterone 27 M. SERIO et al. Table 2 Methodologie problems in competitive binding methods for androgens Binding proteins Labeled steroid Conditions of a s s a y Separation of bound and unbound fraction Extraction of androgens from the plasma and removal of interfering factors Separation of competing steroids plasma proteins antibodies specific activity radiochemical purity isotope time buffer pH temperature gel filtration (Sephadex) ligand adsorption (dextran coated charcoal, F l o r i s i l , e t c .
The impure gamma globulin (without antibodies) bound 10% of added testosterone-1,2- H. 37 M. SERI0 et al. 0 p H Fig. 8. - Effects of pH variations on bound % of plasma testerone radioassay (mean of triplicate determinations). p ε CO M. S E R I O et al. bound 0 IS SO *x> TESTOSTERONE (Pfl) Fig. 10. - Effects of Sephadex LH 20 eluates (curve G) on calibration curve of testosterone radioassay. 40 Problems Connected with Steroid Radioimmunoassay P . G . Crosignani*, R. Malvano**, A. Massaglia**, P.
Lieberman, J. Biol. Chem. 228, 713 (1958). B . F . M. Beiser, F . Borek, F . Edel, S. Lieberman, in "Methods in Immunology and immunoc h e m i s t r y " , E . A . Williams a n d M . C . Chase, Eds. vol 1. Academic P r e s s , New York (1967). G. Scatchard, Ann. N . Y . Acad. Sei. 5 1 , 660 (1949). R. Sips, J. Chem. Phys. 16, 490(1948). U. Barbieri, A. Massaglia, M. Zannino and U. Rosa. J. Chromatography (in p r e s s ) . A. MassagUa, C. Siri-Upathum, M. Zannino and R. Vitali, " I-labelled Steroid TME Derivatives for RadioImmunoas say "(submitted to Steroids), 1972.
Aircraft Profile No. 23: The Messerschmitt Bf 110 by Martin C. Windrow